This is what I just did with my latest Shepard. I call it "Kill all male squadmate" trilogy playthrough.
Honey Shepard has just completed the suicide mission.
Garrus died because the Normandy had no shields
Jacob died in the vent because he volunteered for it and I wasn't going to deny him from doing it
Jack escorted the crew back to the ship
Had Thane do the barrier which allowed Grunt to be taken by the seeker swarms
Zaeed was killed as 2nd fireteam leader
Took Samara and Miranda to fight the reaper and lived.
I had Thane and Mordin, both unloyal, holding the line and surprisingly, Thane survives. He will pay for that in ME3.
Jack, MIranda and Samara are going to ME3 and Kasumi and Tali will join them when I recruit them.
Last thing. I gave the Geth to Cerberus.
So now, Honey Shepard has to worry about killing 3 male squadmates instead of 2, no thanks to Thane, when she starts ME3 later today.
Now my question(s) are How is it Thane was able to survive? I did the same except I had Jacob and Mordin holding the line and Jacob survives.
Now I had Jacob escort the crew back instead of Jack leaving her to hold the line with Mordin and both died even though Jack is loyal. How is it she dies and Thane or Jacob survive, if both are unloyal, holding the line? I also did this with Samara, who was loyal, and she to died. So, I'm just what-the-heck is going on. But that's alright because I know at the end of ME3 all male squadmates will be dead which is the goal for this trilogy playthrough.