Xilizhra wrote...
It means she has negative feelings towards some others because of species, nothing more.
Maybe. I think it's an extension of her natural suspicion due to her more cynical (or "realistic," depending on your point of view) attitudes compared to, say, Kaidan or paragon Shepard. You'll notice that she's more cynical about politicians too, compared to Kaidan. But there do seem to be some instances where she feels negatively towards some others simply because of species; I don't think I can disagree with that. I don't see it as a predominant character trait, though, given the other ways in which she cooperates with aliens and disagrees with Terra Firma. I guess it means she's not a one-note character. She has her suspicions and some prejudices, but on the whole she's nowhere near the actual worst examples of speciesism in the game.
Xilizhra wrote...
Do you believe it's positive or negative? And what do you believe she was actually talking about?
Good question. It's important to examine one's cognitive bias, right?

We're talking about Shepard insisting that the other races won't necessarily abandon humanity to save themselves, I believe? Well, I tend towards the paragon approach, since I think that the races of the galaxy need to stand united against the Reapers or they'll just deplete their strength fighting each other and make the extinction of galactic civilization easier to accomplish. Accordingly, I side with non-renegade Shepard on this subject (though, actually, I've never pressed the renegade responses, so I don't know what RenShep says; I just know that I find the paragon and neutral responses fine). But that's just the point of view of Shep and myself. Ashley's is different. She thinks that the practical thing to do is prepare to be abandoned by other races. In some ways, I can't even fault that: it's good to have back-up plans in case elements that one can't control (e.g. other species' governments) don't give one the results one wants. Personally, I'd tend to prioritise doing whatever I could to make cooperation a reality. I can understand Ashley's perspective, though. It's just more cynical, both when it comes to humans and aliens. It's just that humanity is Ashley's own species, so she's automatically on their side. She'll do what it takes to help them survive even if aliens don't help, and I reckon she expects them to do the same.
xxSgt_Reed_24xx wrote...
who does she even call a bug-eyed monster?
Probably well-ninja'd by now, but she doesn't refer to anyone specific. It's more of a spin she puts on a conversation about Liara. She's concerned about Shep's relationship with Liara, but, on the other hand, she speculates that it might be part of an overall diplomatic effort to get close to other species (otherwise known as the bug-eyed monsters, which, yes, is rather prejudiced in my opinion, even though I think she's being flippant).