- Cole is basically just a spirit of Compassion who cast Mirror Image on the dying/dead human mage Cole, so he could only be a virgin, unless he were made more human and it were possible for him. Do you have to make him more human to get the line, in addition to bringing him? I reckon that Imshael is similar, except that he possessed a human as a Desire demon/Choice spirit, rather than made himself it their image.
- Josephine is probably not a virgin, since she and Leliana went to "a real party" or whatever they called it.
- Leliana probably isn't a virgin in any game. Except maybe before the Leliana's Song DLC, unless Marjolaine didn't just tease her with sex, but actually did it with her - that was never clear, IIRC.
- Cullen IS a virgin, prior to his romance.
- Varric probably isn't a virgin, since he probably did it with Bianca.
- In Cassandra's romance, we find out that she had had sex with Regalyan D'Marcall - most likely after the events of Dawn of the Seeker.
- The Iron Bull is obviously not a virgin.
- Dorian might be a virgin prior to romance, since it's probably really hard for a homosexual person to find another willing... participant, I guess, in the Imperium.
- Vivienne is not a virgin, since she is/was Duke Bastien's mistress, which implies they did it.
- Solas implies that he is a virgin, or at least hasn't done it in a long time.
- Blackwall is probably not a virgin, but it's hard to say if he never tells the player. We know he wouldn't hit on a woman, even if she were feeling vulnerable.
- Sera implies she has dated people before, which means she is unlikely to be a virgin.