The famous 'reach and flexibility' quote is probably the one I liked the most; had me paralyzed with laughter, I wasn't expecting *Garrus* to say something like that...that, and the entire conversation between Valley Girl Quarian and Friend Zone Turian.
Also:
Tupari Machine: Nine out of ten Tupari drinkers recommend it to their friends! The last one is ON MY LIST!
Shepard: The Normandy was destroyed; I ended up spaced.
Wrex: Well, you look good. Ah, the benefits of a redundant nervous system, eh?
Shepard: Yeah, humans don't have those...
Wrex: Oh. Well, then, it must have been very painful for you, then.
Joker: Commander, can we shut this thing [EDI] off?
Shepard: If you don't want to hear it, turn the damn sound off.
Joker: That doesn't change anything, it's still watching. Like some creepy kid staring at the back of your head in comp sci, and you just want to punch him, but you can't 'cause he's *special* and sets fires or something...
Grunt: [...] I get it! The enemy was destroyed to punish them all and send a message. I get it; I hate turians. I thought you'd be glad.
Shepard: Yeah, I don't think I'll be coming down here anymore.
Half of what Grunt says is pure gold. He's hopping up and down with glee every time there's a fight, and every time there's not, he's eagerly telling my FemShep about his imprints, like a young boy talking to his mom.